Anti-Aquaporin-5/AQP5 Polyclonal Antibody is a Rabbit antibody targeting Aquaporin-5/AQP5. Anti-Aquaporin-5/AQP5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Pig)

1. Sample:
Eye (Rat) Lysate at 40 μg
Primary: Anti-Aquaporin 5 (TMAB-00139) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
2. Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Aquaporin Polyclonal Antibody, Unconjugated (TMAB-00139) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Aquaporin 5

Human