Anti-Aquaporin-3/AQP3 Polyclonal Antibody is a Rabbit antibody targeting Aquaporin-3/AQP3. Anti-Aquaporin-3/AQP3 Polyclonal Antibody can be used in FCM.

IgG

Human (predicted:Mouse,Rat)

1. Blank control (black line): A431. Primary Antibody (green line): Rabbit Anti-AQP3 antibody (TMAB-00136)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-AQP3 antibody (TMAB-00136)
Dilution: 3 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

FCM: 2ug/Test

Polyclonal

KLH conjugated synthetic peptide: mouse AQP3

Mouse

AQP3

Aquaporin-3

Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism.