Anti-Apolipoprotein E/APOE3 Polyclonal Antibody is a Rabbit antibody targeting Apolipoprotein E/APOE3. Anti-Apolipoprotein E/APOE3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Pig,Cow)

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-APOE3 Polyclonal Antibody, Unconjugated (TMAB-00133) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-APOE3 Polyclonal Antibody, Unconjugated (TMAB-00133) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus.
3. Sample:
Plasma (Mouse) Lysate at 40 μg
Primary: Anti-Apolipoprotein E3 (TMAB-00133) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
4. Sample:
HepG2 (Human) Cell Lysate at 30 μg
Primary: Anti-Apolipoprotein E3 (TMAB-00133) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 54 kDa
5. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Cerebrum (Rat) Lysate at 40 μg
Primary: Anti-Apolipoprotein E3 (TMAB-00133) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 49 kDa
6. Sample:
Lane 1: Mouse Cerebellum Lysates
Lane 2: Mouse Brain Lysates
Primary: Anti-Apolipoprotein E3 (TMAB-00133) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 35 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human APOE3

Human

Metabolism of lipids and lipoproteins,Lipid transport,Cholesterol Metabolism,Lipoproteins/Apolipoproteins,Endoderm,Cholesterol Metabolism,Lipid metabolism,Heart disease,Neurodegenerative disease,Alzheimer's disease,Neurodegenerative disease,Lipid metabolism,Endoderm