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Anti-APOA1 Antibody (5L985) 是一种 Rabbit 抗体,靶向 APOA1。Anti-APOA1 Antibody (5L985) 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-APOA1 Antibody (5L985) 是一种 Rabbit 抗体,靶向 APOA1。Anti-APOA1 Antibody (5L985) 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 25 μL | ¥ 790 | 5日内发货 | |
| 50 μL | ¥ 1,395 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
| 产品描述 | Anti-APOA1 Antibody (5L985) is a Rabbit antibody targeting APOA1. Anti-APOA1 Antibody (5L985) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 克隆号 | 5L985 |
| 反应种属 | Human,Mouse |
| 验证活性 | 1. Western blot analysis of HepG2 cell lysate. Using APOA1 (TMAB-02753) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 2. 25 μg total protein per lane of various lysates (see on figure) probed with APOA1 monoclonal antibody, unconjugated (TMAB-02753) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 3. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with APOA1 Monoclonal Antibody, Unconjugated (TMAB-02753) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with APOA1 Monoclonal Antibody, Unconjugated (TMAB-02753) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. |
| 应用 | WBIHC-PIHC-FrIF |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Secreted. |
| 组织特异性 | Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 研究背景 | Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. |
| 免疫原 | A synthesized peptide: human Apolipoprotein A1 |
| 抗原种属 | Human |
| 基因名称 | APOA1 |
| 基因ID | |
| 蛋白名称 | Apolipoprotein A-I |
| Uniprot ID | |
| 功能 | Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. |
| 分子量 | Theoretical: 31 kDa. Actual: 31 kDa. |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |