Anti-API2/BIRC3 Antibody (3Y355) is a Rabbit antibody targeting API2/BIRC3. Anti-API2/BIRC3 Antibody (3Y355) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,IP.

IgG

3Y355

Human

1. 25 μg total protein per lane of various lysates (see on figure) probed with API2/BIRC3 monoclonal antibody, unconjugated (TMAB-02746) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with API2/BIRC3 Monoclonal Antibody, Unconjugated (TMAB-02746) at 1:100 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Rhabdomyosarcoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with API2/BIRC3 Monoclonal Antibody, Unconjugated (TMAB-02746) at 1:100 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with API2/BIRC3 Monoclonal Antibody, Unconjugated (TMAB-02746) at 1:100 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with API2/BIRC3 Monoclonal Antibody, Unconjugated (TMAB-02746) at 1:100 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with API2/BIRC3 Monoclonal Antibody, Unconjugated (TMAB-02746) at 1:100 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with API2/BIRC3 Monoclonal Antibody, Unconjugated (TMAB-02746) at 1:100 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-100; IF: 1:50-200; IP: 1:20-50

Monoclonal

A synthesized peptide: human BIRC3

Human

BIRC3

Baculoviral IAP repeat-containing protein 3

Apoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2).