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Anti-APCDD1 Polyclonal Antibody
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产品编号 TMAB-00127
Anti-APCDD1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 APCDD1。Anti-APCDD1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-APCDD1 Polyclonal Antibody
Anti-APCDD1 Polyclonal Antibody
还可以产品编号 TMAB-00127
Anti-APCDD1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 APCDD1。Anti-APCDD1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,160 | 5日内发货 | |
| 100 μL | ¥ 1,970 | 5日内发货 | |
| 200 μL | ¥ 2,795 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-APCDD1 Polyclonal Antibody is a Rabbit antibody targeting APCDD1. Anti-APCDD1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| 交叉反应 | Human (predicted:Mouse,Rat,Chicken,Dog,Rabbit) |
| 验证活性 | 1. Formalin-fixed and paraffin embedded human stomach labeled with Rabbit Anti-APCDD1 Polyclonal Antibody (TMAB-00127) at 1:250 room temperature overnight followed by conjugation to secondary antibody. 2. Sample: DU145 Cell (Human) Lysate at 40 μg Primary: Anti-APCDD1 (TMAB-00127) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kDa Observed band size: 56 kDa 3. Paraformaldehyde-fixed, paraffin embedded (Human pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (APCDD1) Polyclonal Antibody, Unconjugated (TMAB-00127) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cell membrane; Single-pass type I membrane protein. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | APCDD1 is a novel protein that has been shown to be a target of Wnt/beta catenin signaling pathway in cancer cell lines. APCDD1 is overexpressed in colorectal carcinogenesis and is deregulated in CTNNB1 mutated Wilms tumors. [FUNCTION] Probably plays a role in colorectal tumorigenesis. May be a developmental target gene of the Wnt/Beta-catenin pathway. [SUBCELLULAR LOCATION] Membrane; Single-pass type I membrane protein (Potential). [TISSUE SPECIFICITY] Abundantly expressed in heart, pancreas, prostate and ovary. Moderately expressed in lung, liver, kidney, spleen, thymus, colon and peripheral lymphocytes. [INDUCTION] Transcriptionally regulated by the CTNNB1/TF7L2complex. |
| 免疫原 | KLH conjugated synthetic peptide: human APCDD1 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Signal transduction,Surface Molecules |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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