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Anti-ANGPT2/Angiopoietin-2 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 ANGPT2/Angiopoietin-2。Anti-ANGPT2/Angiopoietin-2 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-ANGPT2/Angiopoietin-2 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 ANGPT2/Angiopoietin-2。Anti-ANGPT2/Angiopoietin-2 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,165 | 5日内发货 | |
100 μL | ¥ 1,975 | 5日内发货 | |
200 μL | ¥ 2,785 | 5日内发货 |
产品描述 | Anti-ANGPT2/Angiopoietin-2 Polyclonal Antibody is a Rabbit antibody targeting ANGPT2/Angiopoietin-2. Anti-ANGPT2/Angiopoietin-2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
交叉反应 | Human (predicted:Mouse,Rat,Chicken,Dog,Pig) |
验证活性 | 1. Sample: HepG2 (Human) Cell Lysate at 30 μg Primary: Anti-Angiopoietin 2 (TMAB-00120) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kDa Observed band size: 53 kDa 2. Tissue/cell: human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Ang2 Polyclonal Antibody, Unconjugated (TMAB-00120) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Secreted. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
免疫原 | KLH conjugated synthetic peptide: human Angiopoietin 2 |
抗原种属 | Human |
基因ID | |
Uniprot ID |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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