Anti-Angiotensinogen Polyclonal Antibody 3 is a Rabbit antibody targeting Angiotensinogen. Anti-Angiotensinogen Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human)

1. Sample:
Lane 1: Plasma (Mouse) at 20 μg
Lane 2: Kidney (Mouse) Lysate at 40 μg
Lane 3: Adrenal gland (Mouse) Lysate at 40 μg
Lane 4: Spleen (Rat) Lysate at 40 μg
Lane 5: Plasma (Rat) at 20 μg
Primary: Anti-Angiotensinogen (TMAB-02555) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50-70 kD
Observed band size: 60 kD
2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Angiotensinogen) Polyclonal Antibody, Unconjugated (TMAB-02555) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: mouse Angiotensinogen

Mouse

AGT

Angiotensinogen

Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.Angiotensin-2: acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.Angiotensin-3: stimulates aldosterone release.Angiotensin 1-7: is a ligand for the G-protein coupled receptor MAS1. Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.