Anti-ALS2CR2 Polyclonal Antibody is a Rabbit antibody targeting ALS2CR2. Anti-ALS2CR2 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse,Rat

1. Blank control: SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-ALS2CR2 antibody (TMAB-02499)
Dilution: 2 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Rat Heart tissue lysates
Primary: Anti-ALS2CR2 (TMAB-02499) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 47 kDa

WB: 1:500-2000; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human ALS2CR2

Human

STRADB

STE20-related kinase adapter protein beta