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Anti-Alpha-fetoprotein Antibody (9O812) 是一种 Rabbit 抗体,靶向 Alpha-fetoprotein。Anti-Alpha-fetoprotein Antibody (9O812) 可用于 WB,ELISA,IP。
Anti-Alpha-fetoprotein Antibody (9O812) 是一种 Rabbit 抗体,靶向 Alpha-fetoprotein。Anti-Alpha-fetoprotein Antibody (9O812) 可用于 WB,ELISA,IP。
产品描述 | Anti-Alpha-fetoprotein Antibody (9O812) is a Rabbit antibody targeting Alpha-fetoprotein. Anti-Alpha-fetoprotein Antibody (9O812) can be used in WB,ELISA,IP. |
Ig Type | Monoclonal Rabbit IgG |
克隆号 | 9O812 |
交叉反应 | Human |
验证活性 | 1. AFP was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate. -2 µL anti-AFP rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G. -Primary antibody: -Anti-AFP rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 68 kDa. -Observed band size: 68 kDa. 2. Anti-AFP rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:69 kDa. -Observed band size:69 kDa |
应用 | WB,ELISA,IP |
推荐剂量 | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 0.5-2 μL/mg of lysate |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human AFP / alpha-fetoprotein (rh AFP / alpha-fetoprotein; TMPY-02509; P02771; Met1-Val609). |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
研究背景 | Alpha-fetoprotein (AFP) is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitamin D (Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is one of the several embryo-specific proteins and is a dominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver(1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
偶联 | Unconjugated |
免疫原 | Recombinant Human AFP / alpha-fetoprotein protein (TMPY-02509) |
抗原种属 | Human |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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