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Anti-ALDOA Antibody (1Q763) 是一种 Rabbit 抗体,靶向 ALDOA。Anti-ALDOA Antibody (1Q763) 可用于 WB,ICC,IF,IHC。
Anti-ALDOA Antibody (1Q763) 是一种 Rabbit 抗体,靶向 ALDOA。Anti-ALDOA Antibody (1Q763) 可用于 WB,ICC,IF,IHC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,495 | 5日内发货 | |
100 μL | ¥ 2,495 | 5日内发货 |
产品描述 | Anti-ALDOA Antibody (1Q763) is a Rabbit antibody targeting ALDOA. Anti-ALDOA Antibody (1Q763) can be used in WB,ICC,IF,IHC. |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat,zebrafish |
验证活性 | 1. Western blot analysis of Aldolase on different lysates using anti-Aldolase antibody at 1/1,000 dilution. Positive control: Lane 1: A549, Lane 2: Hela, Lane 3: Mouse liver, Lane 4: Rat liver, Lane 5: Rat spleen. 2. Western blot analysis of Aldolase on Zebrafish tissue lysates using anti-Aldolase antibody at 1/200 dilution. 3. Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-Aldolase antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Aldolase antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Aldolase antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Aldolase antibody. Counter stained with hematoxylin. 7. ICC staining Aldolase in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining Aldolase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining Aldolase in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; ICC:IF: 1:50-200 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Fructose 1,6-bisphosphate aldolase catalyses the reversible condensation of glycerone-P and glyceraldehyde 3-phosphate into fructose 1,6-bisphosphate. Fructose 1,6-bisphosphate aldolase exists as three forms: the muscle-specific Aldolase A; the liver-specific Aldolase B; and the brain-specific Aldolase C. Aldolase A, B and C arose from a common ancestral gene from which Aldo-lase B first diverged. Aldolase A is one of the most highly-conserved enzymes known, with only about 2% of the residues changing per 100 million years. Aldolase B is regulated by the hormones insulin and glucagon, and has been implicated in hereditary fructose intolerance disease. Aldolase C is a polypeptide that is exclusively expressed in Purkinje cells. Aldolase C-positive Purkinje cells are organized in the cerebellum as stripes or bands that run from anterior to posterior across the cerebellum and alternate with bands of Aldolase C-negative Purkinje cells. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 39 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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