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Anti-ALDH7A1 Antibody (3P955)

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产品编号 TMAY-01934

Anti-ALDH7A1 Antibody (3P955) 是一种 Rabbit 抗体,靶向 ALDH7A1。Anti-ALDH7A1 Antibody (3P955) 可用于 WB,ELISA,IP。

Anti-ALDH7A1 Antibody (3P955)

Anti-ALDH7A1 Antibody (3P955)

Rating icon 还可以
产品编号 TMAY-01934

Anti-ALDH7A1 Antibody (3P955) 是一种 Rabbit 抗体,靶向 ALDH7A1。Anti-ALDH7A1 Antibody (3P955) 可用于 WB,ELISA,IP。

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产品介绍

生物活性
产品描述
Anti-ALDH7A1 Antibody (3P955) is a Rabbit antibody targeting ALDH7A1. Anti-ALDH7A1 Antibody (3P955) can be used in WB,ELISA,IP.
Ig Type
Monoclonal Rabbit IgG
克隆号
3P955
交叉反应
Human
验证活性
1. Anti-ALDH7A1 rabbit monoclonal antibody at 1:1000 dilution.

-Lane A: Hela Whole Cell Lysate.

-Lane B: HepG2 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:58 kDa.

-Observed band size:58 kDa.

2. ALDH7A1 was immunoprecipitated using:

-Lane A:0.5 mg Hela Whole Cell Lysate.

-2 µL anti-ALDH7A1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-ALDH7A1 rabbit monoclonal antibody, at 1:100 dilution.

-Secondary antibody:

-Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution.

-Developed using the DAB staining technique.

-Performed under reducing conditions.

-Predicted band size: 55 kDa.

-Observed band size: 55 kDa
应用
WB,ELISA,IP
推荐剂量
WB: 1:1000-1:5000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human ALDH7A1 (rh ALDH7A1; TMPY-01588; P49419-2; Ser 2-Gln 511).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景ALDH7A1 (Aldehyde dehydrogenase 7 family, member A1) is a member of subfamily 7 in the aldehyde dehydrogenase family. These enzymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. Mammalian ALDH7A1 is homologous to plant ALDH7B1 which protects against various forms of stress such as increased salinity, dehydration and treatment with oxidants or pesticides. In mammals, ALDH7A1 is known to play a primary role during lysine catabolism through the NAD+-dependent oxidative conversion of aminoadipate semialdehyde (AASA) to its corresponding carboxylic acid, α-aminoadipic acid. Deleterious mutations in human ALDH7A1 are responsible for pyridoxine-dependent and folinic acid-responsive seizures. ALDH7A1 is a novel aldehyde dehydrogenase expressed in multiple subcellular compartments that protects against hyperosmotic stress by generating osmolytes and metabolizing toxic aldehydes.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Human ALDH7A1 protein (TMPY-01588)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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