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顶部生物活性储存和溶解信息计算器

Anti-ALDH4A1 Antibody (6Q90)

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Anti-ALDH4A1 Antibody (6Q90)
产品编号 TMAY-02423
别名 P5CDh, P5CD, ALDH4, aldehyde dehydrogenase 4 family, member A1

Anti-ALDH4A1 Antibody (6Q90) 是一种 Rabbit 抗体，靶向 ALDH4A1。Anti-ALDH4A1 Antibody (6Q90) 可用于 WB,ELISA,IP。

Anti-ALDH4A1 Antibody (6Q90)
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Anti-ALDH4A1 Antibody (6Q90)

Rating icon 还可以
Anti-ALDH4A1 Antibody (6Q90)
产品编号 TMAY-02423 别名 P5CDh, P5CD, ALDH4, aldehyde dehydrogenase 4 family, member A1

Anti-ALDH4A1 Antibody (6Q90) 是一种 Rabbit 抗体，靶向 ALDH4A1。Anti-ALDH4A1 Antibody (6Q90) 可用于 WB,ELISA,IP。

规格价格库存数量
100 μL
¥ 2,294
5日内发货
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产品介绍

生物活性
产品描述
Anti-ALDH4A1 Antibody (6Q90) is a Rabbit antibody targeting ALDH4A1. Anti-ALDH4A1 Antibody (6Q90) can be used in WB,ELISA,IP.
别名P5CDh, P5CD, ALDH4, aldehyde dehydrogenase 4 family, member A1
Ig Type
Rabbit IgG
克隆号
6Q90
交叉反应
Human
验证活性
1. Anti-ALDH4A1 rabbit monoclonal antibody at 1:500 dilution.
-Lane A: HepG2 Whole Cell Lysate.
-Lane B: K562 Whole Cell Lysate.
-Lane C: A549 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:62 kDa.
-Observed band size:62 kDa(We are unsure as to the identity of these extra bands.)
2. ALDH4A1 was immunoprecipitated using:
-Lane A:0.5 mg Jurkat Whole Cell Lysate.
-Lane B:0.5 mg K562 Whole Cell Lysate.
-2 µL anti-ALDH4A1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-ALDH4A1 rabbit monoclonal antibody, at 1:200 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 62 kDa.
-Observed band size: 62 kDa
应用
WB,ELISA,IP
推荐剂量
WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human ALDH4A1 (rh ALDH4A1; TMPY-02799; AAH07581.1; Lys25-Gln563).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景ALDH4A1 is a member of the aldehyde dehydrogenase family. Aldehyde dehydrogenase enzymes function in the metabolism of many molecules including certain fats (cholesterol and other fatty acids) and protein building blocks (amino acids). Additional aldehyde dehydrogenase enzymes detoxify external substances, such as alcohol and pollutants, and internal substances, such as toxins that are formed within cells. ALDH4A1 is expressed abundantly in liver followed by skeletal muscle, kidney, heart, brain, placenta, lung and pancreas. It is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2). HP-2 is characterized by the accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. The disorder may be causally related to neurologic manifestations, including seizures and mental retardation.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Human ALDH4A1 Protein (TMPY-02799)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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关键词

ALDH-4ALDH 4

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