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Anti-ALDH4A1 Antibody (6Q90) 是一种 Rabbit 抗体,靶向 ALDH4A1。Anti-ALDH4A1 Antibody (6Q90) 可用于 WB,ELISA,IP。
Anti-ALDH4A1 Antibody (6Q90) 是一种 Rabbit 抗体,靶向 ALDH4A1。Anti-ALDH4A1 Antibody (6Q90) 可用于 WB,ELISA,IP。
产品描述 | Anti-ALDH4A1 Antibody (6Q90) is a Rabbit antibody targeting ALDH4A1. Anti-ALDH4A1 Antibody (6Q90) can be used in WB,ELISA,IP. |
Ig Type | Monoclonal Rabbit IgG |
克隆号 | 6Q90 |
交叉反应 | Human |
验证活性 | 1. Anti-ALDH4A1 rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell Lysate. -Lane B: K562 Whole Cell Lysate. -Lane C: A549 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:62 kDa. -Observed band size:62 kDa(We are unsure as to the identity of these extra bands.) 2. ALDH4A1 was immunoprecipitated using: -Lane A:0.5 mg Jurkat Whole Cell Lysate. -Lane B:0.5 mg K562 Whole Cell Lysate. -2 µL anti-ALDH4A1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-ALDH4A1 rabbit monoclonal antibody, at 1:200 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 62 kDa. -Observed band size: 62 kDa |
应用 | WB,ELISA,IP |
推荐剂量 | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human ALDH4A1 (rh ALDH4A1; TMPY-02799; AAH07581.1; Lys25-Gln563). |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
研究背景 | ALDH4A1 is a member of the aldehyde dehydrogenase family. Aldehyde dehydrogenase enzymes function in the metabolism of many molecules including certain fats (cholesterol and other fatty acids) and protein building blocks (amino acids). Additional aldehyde dehydrogenase enzymes detoxify external substances, such as alcohol and pollutants, and internal substances, such as toxins that are formed within cells. ALDH4A1 is expressed abundantly in liver followed by skeletal muscle, kidney, heart, brain, placenta, lung and pancreas. It is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2). HP-2 is characterized by the accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. The disorder may be causally related to neurologic manifestations, including seizures and mental retardation. |
偶联 | Unconjugated |
免疫原 | Recombinant Human ALDH4A1 Protein (TMPY-02799) |
抗原种属 | Human |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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