Anti-ALDH1A1 Polyclonal Antibody is a Rabbit antibody targeting ALDH1A1. Anti-ALDH1A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Cow,Horse,Rabbit,Sheep,Pig)

1. Sample: Placenta (Mouse) Lysate at 40 μg
Primary: Anti-ALDH1 (TMAB-00092) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
2. Paraformaldehyde-fixed, paraffin embedded (human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ALDH1) Polyclonal Antibody, Unconjugated (TMAB-00092) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ALDH1) Polyclonal Antibody, Unconjugated (TMAB-00092) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
4. Sample:
A549 Cell (Human) Lysate at 40 μg
HepG2 Cell (Human) Lysate at 40 μg
Primary: Anti-ALDH1 (TMAB-00092) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
5. Sample: Liver (mouse) Lysate at 40 μg
Primary: Anti-ALDH1 (TMAB-00092) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human ALDH1

Human

Tumor biomarkers,Soma marker,Parkinson's disease,Neurogenesis,Intracellular Molecules