Anti-ALCAM Antibody (3O178) is an antibody targeting ALCAM. Anti-ALCAM Antibody (3O178) can be used in ELISA, WB, IHC.

Rabbit IgG

3O178

Human, Mouse

1. Western Blot
-Positive WB detected in: PC3 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate, Ntera-2 whole cell lysate, Mouse brain tissue
-All lanes: ALCAM antibody at 1:2000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 66, 64, 16, 34 kDa
-Observed band size: 100 kDa
2. IHC image of TMAH-00051 diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
3. IHC image of TMAH-00051 diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

ELISA, WB, IHC

WB:1:500-1:2000; IHC:1:50-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human ALCAM

Human

214

Tags & Cell Markers, Immunology