Anti-Albumin Polyclonal Antibody is a Rabbit antibody targeting Albumin. Anti-Albumin Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse

1. Sample:
A549 (Human) Cell Lysate at 30 μg
Primary: Anti-Mouse serum albumin (TMAB-00091) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
2. Sample:
Lung (Mouse) Lysate at 40 μg
Spleen (Mouse) Lysate at 40 μg
Primary: Anti-Mouse serum albumin (TMAB-00091) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
3. Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Mouse serum albumin) Polyclonal Antibody, Unconjugated (TMAB-00091) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 min and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (Mouse Liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Mouse serum albumin) Polyclonal Antibody, Unconjugated (TMAB-00091) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 min and DAB staining.
5. Sample:
Lane 1: Mouse Serum
Lane 2: Human Serum
Primary: Anti-Albumin (TMAB-00091) at 1/5000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 62 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

Mouse serum albumin purified from mouse serum

Mouse

SERPINB14

Ovalbumin

Endoderm,Other Chaperones,Lipid metabolism,Metabolic disorders,Signal transduction proteins ELISA kits,Endoderm,Serum Proteins

Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.