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Anti-ALAS1 Antibody (7J767)

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Anti-ALAS1 Antibody (7J767)
产品编号 TMAC-00130
别名 nonspecific, mitochondrial, EC 2.3.1.37, Delta-aminolevulinate synthase 1, Delta-ALA synthase 1, ALAS-H, ALAS3 ALASH, ALAS 1, 5-aminolevulinic acid synthase 1, 5-aminolevulinate synthase

Anti-ALAS1 Antibody (7J767) 是一种 Rabbit 抗体，靶向 ALAS1。Anti-ALAS1 Antibody (7J767) 可用于 WB,ICC/IF。

Anti-ALAS1 Antibody (7J767)

Anti-ALAS1 Antibody (7J767)

Rating icon 还可以
Anti-ALAS1 Antibody (7J767)
产品编号 TMAC-00130 别名 nonspecific, mitochondrial, EC 2.3.1.37, Delta-aminolevulinate synthase 1, Delta-ALA synthase 1, ALAS-H, ALAS3 ALASH, ALAS 1, 5-aminolevulinic acid synthase 1, 5-aminolevulinate synthase

Anti-ALAS1 Antibody (7J767) 是一种 Rabbit 抗体，靶向 ALAS1。Anti-ALAS1 Antibody (7J767) 可用于 WB,ICC/IF。

规格价格库存数量
50 μL
¥ 1,480
5日内发货
100 μL
¥ 2,485
5日内发货
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产品介绍

生物活性
产品描述
Anti-ALAS1 Antibody (7J767) is a Rabbit antibody targeting ALAS1. Anti-ALAS1 Antibody (7J767) can be used in WB,ICC/IF.
别名nonspecific, mitochondrial, EC 2.3.1.37, Delta-aminolevulinate synthase 1, Delta-ALA synthase 1, ALAS-H, ALAS3 ALASH, ALAS 1, 5-aminolevulinic acid synthase 1, 5-aminolevulinate synthase
Ig Type
IgG
交叉反应
Human
验证活性
1. Western blot analysis of Alas1 on different cell lysates using anti-Alas1 antibody at 1/1,000 dilution. Positive control: Lane 1: JAR, Lane 2: HUVEC, Lane 3: Hela.
2. ICC staining Alas1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
3. ICC staining Alas1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining Alas1 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
应用
WB
ICC/IF
推荐剂量
WB: 1:1000-5000; ICC/IF: 1:50-200
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals. The erythroid-specific isoenzyme, ALAS-E, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver. ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells. Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H. The ALAS-E 5'-flanking region contains binding sites for nuclear activators such as GATA-1, NF-E2 and EKLF. Since the ALAS gene maps to the X chromosome, mutation of the gene leads to the pyridoxine-refractory X-linked sideroblastic anemia.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
P13196
化学信息
分子量Theoretical: 71 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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