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Anti-AKAP12 Polyclonal Antibody

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货号 TMAB-00084

别名 Tsga12, SSeCKS, Srcs5, Myasthenia gravis autoantigen gravin, Kinase scaffold protein gravin, Gravin, Gercelin, DKFZp686O0331, DKFZp686M0430, AKAP250, AKAP 250, AKAP 12, A kinase(PRKA) anchor protein(gravin) 12, A kinase anchor protein 250kDa, A kinase Anchor Protein 12

Anti-AKAP12 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 AKAP12。Anti-AKAP12 Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P,WB。

Anti-AKAP12 Polyclonal Antibody

Anti-AKAP12 Polyclonal Antibody

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货号 TMAB-00084 别名 Tsga12, SSeCKS, Srcs5, Myasthenia gravis autoantigen gravin, Kinase scaffold protein gravin, Gravin, Gercelin, DKFZp686O0331, DKFZp686M0430, AKAP250, AKAP 250, AKAP 12, A kinase(PRKA) anchor protein(gravin) 12, A kinase anchor protein 250kDa, A kinase Anchor Protein 12

Anti-AKAP12 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 AKAP12。Anti-AKAP12 Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P,WB。

规格价格库存数量
50 μL
¥ 1,160
5日内发货
100 μL
¥ 1,965
5日内发货
200 μL
¥ 2,785
5日内发货
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产品介绍

生物活性
产品描述
Anti-AKAP12 Polyclonal Antibody is a Rabbit antibody targeting AKAP12. Anti-AKAP12 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
别名Tsga12, SSeCKS, Srcs5, Myasthenia gravis autoantigen gravin, Kinase scaffold protein gravin, Gravin, Gercelin, DKFZp686O0331, DKFZp686M0430, AKAP250, AKAP 250, AKAP 12, A kinase(PRKA) anchor protein(gravin) 12, A kinase anchor protein 250kDa, A kinase Anchor Protein 12
Ig Type
IgG
反应种属
Human,Mouse,Rat (predicted:Dog)
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AKAP12) Polyclonal Antibody, Unconjugated (TMAB-00084) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nucleus staining.
2. Sample:
Lane1: mouse bladder Lysate at 25 μg
Lane2: mouse lung Lysate at 25 μg
Primary: Anti-AKAP12 (TMAB-00084) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 191 kDa
Observed band size: 191 kDa
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AKAP12) Polyclonal Antibody, Unconjugated (TMAB-00084) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 90 minutes, and DAPI for nucleus staining.
4. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AKAP12) Polyclonal Antibody, Unconjugated (TMAB-00084) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 min and DAB staining.
verifiedActivityverifiedActivityverifiedActivityverifiedActivity
应用IFIHC-FrIHC-PWB
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:200-800
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cytoplasm
组织特异性Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1 mg/mL
研究背景The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
抗原信息
免疫原
KLH conjugated synthetic peptide: human AKAP12
抗原种属
Human
基因名称
AKAP12
基因ID
9590
蛋白名称
A-kinase anchor protein 12
Uniprot ID
Q02952
研究领域
Neurodegenerative disease,Cytoplasmic
功能
Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
化学信息
分子量Theoretical: 191 kDa.
储存&溶解度
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.
存储store at low temperature | store at -20°C

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