Anti-AKAP12 Polyclonal Antibody is a Rabbit antibody targeting AKAP12. Anti-AKAP12 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Dog)

1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AKAP12) Polyclonal Antibody, Unconjugated (TMAB-00084) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nucleus staining.
2. Sample:
Lane1: mouse bladder Lysate at 25 μg
Lane2: mouse lung Lysate at 25 μg
Primary: Anti-AKAP12 (TMAB-00084) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 191 kDa
Observed band size: 191 kDa
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AKAP12) Polyclonal Antibody, Unconjugated (TMAB-00084) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 90 minutes, and DAPI for nucleus staining.
4. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AKAP12) Polyclonal Antibody, Unconjugated (TMAB-00084) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 min and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:200-800

Polyclonal

KLH conjugated synthetic peptide: human AKAP12

Human

Neurodegenerative disease,Cytoplasmic