Anti-AIF Antibody (2K106)

Name: Anti-AIF Antibody (2K106)
Brand: TargetMol
SKU: TMAH-00044
Rating: 4.5 (1 reviews)

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产品编号 TMAH-00044

别名 Programmed Cell Death Protein 8, PDCD8, Apoptosis-Inducing Factor 1 Mitochondrial, AIFM1, AIF

Anti-AIF Antibody (2K106) 是一种抗体，靶向 AIF。Anti-AIF Antibody (2K106) 可用于 ELISA, WB, IHC。

Anti-AIF Antibody (2K106)

还可以

产品编号 TMAH-00044 别名 Programmed Cell Death Protein 8, PDCD8, Apoptosis-Inducing Factor 1 Mitochondrial, AIFM1, AIF

Anti-AIF Antibody (2K106) 是一种抗体，靶向 AIF。Anti-AIF Antibody (2K106) 可用于 ELISA, WB, IHC。

规格	价格	库存	数量
50 μL	¥ 1,305	5日内发货
100 μL	¥ 2,190	5日内发货

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生物活性

产品描述	Anti-AIF Antibody (2K106) is an antibody targeting AIF. Anti-AIF Antibody (2K106) can be used in ELISA, WB, IHC.
别名	Programmed Cell Death Protein 8, PDCD8, Apoptosis-Inducing Factor 1 Mitochondrial, AIFM1, AIF
Ig Type	Rabbit IgG
克隆号	2K106
交叉反应	Human, Rat
验证活性	1. Western Blot -Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Rat liver tissue, Rat kidney tissue -All lanes: AIFM1 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 67, 36, 29, 27 kDa -Observed band size: 67 kDa 2. IHC image of TMAH-00044 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. IHC image of TMAH-00044 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
应用	ELISA, WB, IHC
推荐剂量	WB:1:500-1:5000; IHC:1:50-1:200.
抗体种类	Monoclonal
亚细胞定位	Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm, perinuclear region.; [Isoform 3]: Mitochondrion intermembrane space. Mitochondrion inner membrane.; [Isoform 4]: Mitochondrion. Cytoplasm, cytosol.; [Isoform 5]: Cytoplasm.
构建方式	Recombinant Antibody
纯化方式	Affinity-chromatography
性状	Liquid
缓冲液	Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景	Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Binds to DNA in a sequence-independent manner. Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates caspase-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. In contrast, participates in normal mitochondrial metabolism. Plays an important role in the regulation of respiratory chain biogenesis by interacting with CHCHD4 and controlling CHCHD4 mitochondrial import. Has NADH oxidoreductase activity. Does not induce nuclear apoptosis. Pro-apoptotic isoform.

偶联与修饰

偶联	Unconjugated

抗原信息

免疫原	A synthetic peptide: Human AIF
抗原种属	Human
基因ID	9131
Uniprot ID	O95831
研究领域	Epigenetics and Nuclear Signaling, Cancer, Cell biology, Metabolism