购物车
  • TargetMol
    您的购物车当前为空
前往下单页继续购物
顶部产品介绍抗原信息化学信息储存和溶解信息计算器

Anti-AFAP1L2 Polyclonal Antibody

一键复制产品信息
Rating icon 还可以
货号 TMAB-02373

Anti-AFAP1L2 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 AFAP1L2。Anti-AFAP1L2 Polyclonal Antibody 可用于 WB,FCM。

Anti-AFAP1L2 Polyclonal Antibody

Anti-AFAP1L2 Polyclonal Antibody

一键复制产品信息
Rating icon 还可以
货号 TMAB-02373

Anti-AFAP1L2 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 AFAP1L2。Anti-AFAP1L2 Polyclonal Antibody 可用于 WB,FCM。

规格价格库存数量
50 μL
¥ 1,175
5日内发货
100 μL
¥ 1,970
5日内发货
200 μL
¥ 2,785
5日内发货
库存状态实时更新，以官网显示为准，现货产品可直接加购物车下单
大包装 & 定制
加入购物车
TargetMol的所有产品仅用作科学研究或药证申报，不能被用于人体，我们不向个人提供产品和服务。请您遵守承诺用途，不得违反法律法规规定用于任何其他用途。
实验操作小课堂
常见问题解答
查看更多
资源下载:
产品操作手册

产品介绍

生物活性
产品描述
Anti-AFAP1L2 Polyclonal Antibody is a Rabbit antibody targeting AFAP1L2. Anti-AFAP1L2 Polyclonal Antibody can be used in WB,FCM.
Ig Type
IgG
反应种属
Mouse (predicted:Human,Rat)
验证活性
1. Sample:
Kidney (Mouse) Lysate at 40 μg
Primary: Anti-AFAP1L2 (TMAB-02373) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 90 kD
Observed band size: 90 kD
2. Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-AFAP1L2 antibody (TMAB-02373)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg /test.
Protocol
The cells were fixed with 70% ethanol (10 min at room temperature) and then were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
应用WBFCM
推荐剂量
WB: 1:500-2000; FCM: 2μg/Test
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cytoplasm.
组织特异性Detected in spleen and thyroid, and at lower levels in kidney, brain, lung and pancreas.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景Actin filament associated protein (AFAP-110) interacts directly with actin filaments through its C-terminal actin-binding domain. AFAP-110 contains additional protein-binding domains as well, and serves as an adaptor protein. By linking signaling molecules to actin filaments, AFAP-110 provides a platform for the preparation of larger signaling complexes, activates Src kinases in response to cellular signals and also directly affects Actin organization as an Actin filament cross-linking protein. AFAP-1L2 (Actin filament-associated protein 1-like 2), also known as XB130, is a 818 amino acid cytoplasmic protein that contains two Pleckstrin homology (PH) domains, which are normally found in proteins involved in intracellular signaling. Like its relative AFAP110, AFAP-1L2 interacts with Src kinase and may play a role in Src-regulated transcription activation. AFAP-1L2 is expressed in thyroid and spleen and can also be detected at lower levels in lung, brain, pancreas and kidney. There are four isoforms of AFAP-1L2 that are produced as a result of alternative splicing events.
抗原信息
免疫原
KLH conjugated synthetic peptide: human AFAP1L2
抗原种属
Human
基因名称
AFAP1L2
基因ID
84632
蛋白名称
Actin filament-associated protein 1-like 2
Uniprot ID
Q8N4X5
功能
May play a role in a signaling cascade by enhancing the kinase activity of SRC. Contributes to SRC-regulated transcription activation.
化学信息
分子量Theoretical: 90 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

  • 稀释 计算器
  • 配液 计算器
Related Tags: buy Anti-AFAP1L2 Polyclonal Antibody | purchase Anti-AFAP1L2 Polyclonal Antibody | Anti-AFAP1L2 Polyclonal Antibody cost | order Anti-AFAP1L2 Polyclonal Antibody | Anti-AFAP1L2 Polyclonal Antibody molecular weight