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Anti-ADRB3 Polyclonal Antibody

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Anti-ADRB3 Polyclonal Antibody
产品编号 TMAB-00072

Anti-ADRB3 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 ADRB3。Anti-ADRB3 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-ADRB3 Polyclonal Antibody

Anti-ADRB3 Polyclonal Antibody

Rating icon 还可以
Anti-ADRB3 Polyclonal Antibody
产品编号 TMAB-00072

Anti-ADRB3 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 ADRB3。Anti-ADRB3 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,170
5日内发货
100 μL
¥ 1,965
5日内发货
200 μL
¥ 2,790
5日内发货
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产品介绍

生物活性
产品描述
Anti-ADRB3 Polyclonal Antibody is a Rabbit antibody targeting ADRB3. Anti-ADRB3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
交叉反应
Human,Rat (predicted:Mouse,Dog,Pig,Sheep)
验证活性
1. Tissue/cell: Rat adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-ADRB3 Polyclonal Antibody, Unconjugated (TMAB-00072) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
K562 (Human) Cell Lysate at 40 μg
Primary: Anti-beta 3 Adrenergic Receptor (TMAB-00072) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 54 kDa
应用
WB
IHC-P
IHC-Fr
IF
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cell membrane; Multi-pass membrane protein.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景beta 3 Adrenergic Receptor is located mainly in adipose tissue and is involved in the regulation of lipolysis and thermogenesis. Beta adrenergic receptors are involved in the epenephrine and norepinephrine-induced activation of adenylate cyclase through the action of G proteins. beta 3 Adrenergic Receptor expression has been documented in adipose tissues, heart, and in smooth muscle of digestive and urinary tract organs (bladder, colon, small intestine, stomach, ureter). Utilization of alternate promoters and/or 3-prime untranslated regions may result in tissue-specific regulation of the expression of this protein.
抗原信息
免疫原
KLH conjugated synthetic peptide: human ADRB3
抗原种属
Human
基因ID
155
Uniprot ID
P13945
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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