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Anti-ADRA2C Polyclonal Antibody

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货号 TMAB-02357

Anti-ADRA2C Polyclonal Antibody 是一种 Rabbit 抗体,靶向 ADRA2C。Anti-ADRA2C Polyclonal Antibody 可用于 WB,FCM。

Anti-ADRA2C Polyclonal Antibody

Anti-ADRA2C Polyclonal Antibody

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Rating icon 还可以
货号 TMAB-02357

Anti-ADRA2C Polyclonal Antibody 是一种 Rabbit 抗体,靶向 ADRA2C。Anti-ADRA2C Polyclonal Antibody 可用于 WB,FCM。

规格价格库存数量
50 μL
¥ 1,170
5日内发货
100 μL
¥ 1,970
5日内发货
200 μL
¥ 2,790
5日内发货
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产品介绍


生物活性
产品描述
Anti-ADRA2C Polyclonal Antibody is a Rabbit antibody targeting ADRA2C. Anti-ADRA2C Polyclonal Antibody can be used in WB,FCM.
Ig Type
IgG
反应种属
Human (predicted:Mouse,Rat,Dog,Pig)
验证活性
1. Blank control (blue): U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-ADRA2C antibody (TMAB-02357), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.
2. Blank control (blue line): A431 (fixed with 70% ice-cold methanol overnight at 4℃).
Primary Antibody (green line): Rabbit Anti-ADRA2C/PE-CY5 Conjugated antibody (TMAB-02357-PE-CY5), Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-PE-CY5.
3. Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human MCF-7 cell lysates
Lane 3: Human SH-SY5Y cell lysate
Lane 4: Human 293T cell lysates
Lane 5: Human A549 cell lysates
Primary: Anti-ADRA2C (TMAB-02357) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 58 kDa
应用WBFCM
推荐剂量
WB: 1:500-2000; FCM: 1μg/Test
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cell membrane; Multi-pass membrane protein.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. The mouse studies revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons. The alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes the alpha2C subtype, which contains no introns in either its coding or untranslated sequences. [provided by RefSeq, Jul 2008]
抗原信息
免疫原
KLH conjugated synthetic peptide: human ADRA2C/alpha 2C Adrenergic Receptor
抗原种属
Human
基因名称
ADRA2C
基因ID
蛋白名称
alpha-2C adrenergic receptor
Uniprot ID
功能
The alpha 2C Adrenergic Receptor controls the release of neurotransmitter from central adrenergic neurons and from sympathetic nerves in the heart. This receptor also plays a role in cognitive and behavioral function. Two variants are produced by alternative splicing. Alpha 2C Adrenergic Receptor has been reported extensively in the brain. Blood, corpus cavermosum, kidney, and heart tissues have also been reported to express the protein.
化学信息
分子量Theoretical: 49 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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