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Anti-ADRA2C Polyclonal Antibody 是一种 Rabbit 抗体,靶向 ADRA2C。Anti-ADRA2C Polyclonal Antibody 可用于 WB,FCM。
Anti-ADRA2C Polyclonal Antibody 是一种 Rabbit 抗体,靶向 ADRA2C。Anti-ADRA2C Polyclonal Antibody 可用于 WB,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,970 | 5日内发货 | |
| 200 μL | ¥ 2,790 | 5日内发货 |
| 产品描述 | Anti-ADRA2C Polyclonal Antibody is a Rabbit antibody targeting ADRA2C. Anti-ADRA2C Polyclonal Antibody can be used in WB,FCM. |
| Ig Type | IgG |
| 反应种属 | Human (predicted:Mouse,Rat,Dog,Pig) |
| 验证活性 | 1. Blank control (blue): U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-ADRA2C antibody (TMAB-02357), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA. 2. Blank control (blue line): A431 (fixed with 70% ice-cold methanol overnight at 4℃). Primary Antibody (green line): Rabbit Anti-ADRA2C/PE-CY5 Conjugated antibody (TMAB-02357-PE-CY5), Dilution: 1 μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-PE-CY5. 3. Sample: Lane 1: Human HepG2 cell lysates Lane 2: Human MCF-7 cell lysates Lane 3: Human SH-SY5Y cell lysate Lane 4: Human 293T cell lysates Lane 5: Human A549 cell lysates Primary: Anti-ADRA2C (TMAB-02357) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kDa Observed band size: 58 kDa |
| 应用 | WBFCM |
| 推荐剂量 | WB: 1:500-2000; FCM: 1μg/Test |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. |
| 构建方式 | Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 浓度 | 1mg/ml |
| 研究背景 | Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. The mouse studies revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons. The alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes the alpha2C subtype, which contains no introns in either its coding or untranslated sequences. [provided by RefSeq, Jul 2008] |
| 免疫原 | KLH conjugated synthetic peptide: human ADRA2C/alpha 2C Adrenergic Receptor |
| 抗原种属 | Human |
| 基因名称 | ADRA2C |
| 基因ID | |
| 蛋白名称 | alpha-2C adrenergic receptor |
| Uniprot ID | |
| 功能 | The alpha 2C Adrenergic Receptor controls the release of neurotransmitter from central adrenergic neurons and from sympathetic nerves in the heart. This receptor also plays a role in cognitive and behavioral function. Two variants are produced by alternative splicing. Alpha 2C Adrenergic Receptor has been reported extensively in the brain. Blood, corpus cavermosum, kidney, and heart tissues have also been reported to express the protein. |
| 分子量 | Theoretical: 49 kDa. |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |