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顶部产品介绍抗原信息化学信息储存和溶解信息计算器

Anti-ADGRE1 Antibody (1T88)

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货号 TMAB-02332

Anti-ADGRE1 Antibody (1T88) 是一种 Rabbit 抗体，靶向 ADGRE1。Anti-ADGRE1 Antibody (1T88) 可用于 FCM,WB。

Anti-ADGRE1 Antibody (1T88)

Anti-ADGRE1 Antibody (1T88)

一键复制产品信息
Rating icon 还可以
货号 TMAB-02332

Anti-ADGRE1 Antibody (1T88) 是一种 Rabbit 抗体，靶向 ADGRE1。Anti-ADGRE1 Antibody (1T88) 可用于 FCM,WB。

规格价格库存数量
25 μL
¥ 785
5日内发货
50 μL
¥ 1,380
5日内发货
100 μL
¥ 2,485
5日内发货
200 μL
¥ 3,980
5日内发货
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产品介绍

生物活性
产品描述
Anti-ADGRE1 Antibody (1T88) is a Rabbit antibody targeting ADGRE1. Anti-ADGRE1 Antibody (1T88) can be used in FCM,WB.
Ig Type
IgG
克隆号
1T88
反应种属
Human
验证活性
1. Blank control: THP-1.
Primary Antibody (green line): Rabbit Anti-ADGRE1 antibody (TMAB-02332)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line) : Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Sample:
Lane 1: Human THP-1 cell lysates
Primary: Anti-ADGRE1 (TMAB-02332) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95 kDa
Observed band size: 100 kDa
应用FCMWB
推荐剂量
FCM: 1μg/Test; WB: 1:500-2000
抗体种类
Monoclonal
宿主来源Rabbit
亚细胞定位Cell membrane.
组织特异性Wide expression; increased levels in peripheral blood mononuclear cells.
构建方式Recombinant Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景The epidermal growth factor (EGF)-TM7 family constitutes a group of class B G-protein coupled receptors, which includes CD97, EMR1 (EGF-like molecule containing mucin-like hormone receptor 1, designated F4/80 in mouse), EMR2, EMR3, FIRE, and ETL (1–3). These family members are characterized by an extended extracellular region with several N-terminal EGF domains, and are predominantly expressed on cells of the immune system (1–3). The EGF-TM7 protein family are encoded by a gene cluster on human chromosome 19p13 (1,3,4). The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria, and Langerhans cells in the skin (1). F4/80/EMR1 undergoes extensive N-linked glycosylation as well as some O-linked glycosylation (5,6). The function of F4/80/EMR1 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration, or as a G-protein coupled signaling component of macrophages.
抗原信息
免疫原
Recombinant Protein: human ADGRE1
抗原种属
Human
基因名称
ADGRE1
基因ID
2015
蛋白名称
Adhesion G protein-coupled receptor E1
Uniprot ID
Q14246
功能
Could be involved in cell-cell interactions.
化学信息
分子量Theoretical: 95 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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