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Anti-Adenylosuccinate Lyase Antibody (8T693) 是一种 Mouse 抗体,靶向 Adenylosuccinate Lyase。Anti-Adenylosuccinate Lyase Antibody (8T693) 可用于 ICC/IF。
Anti-Adenylosuccinate Lyase Antibody (8T693) 是一种 Mouse 抗体,靶向 Adenylosuccinate Lyase。Anti-Adenylosuccinate Lyase Antibody (8T693) 可用于 ICC/IF。
产品描述 | Anti-Adenylosuccinate Lyase Antibody (8T693) is a Mouse antibody targeting Adenylosuccinate Lyase. Anti-Adenylosuccinate Lyase Antibody (8T693) can be used in ICC/IF. |
Ig Type | Monoclonal Mouse IgG1 |
克隆号 | 8T693 |
交叉反应 | Human |
验证活性 | Immunofluorescence staining of His-ADSL in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-Human His-ADSL monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm. |
应用 | ICC/IF |
推荐剂量 | ICC-IF: 1:20-1:100 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | This product is a recombinant monoclonal antibody expressed from HEK293 cells. |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
研究背景 | Adenylosuccinate lyase, also known as adenylosuccinase, ADSL or ASL, is an enzyme implicated in the reaction of adenylosuccinat converting to AMP and fumarate as part of the purine nucleotide cycle. The two substates of adenylosuccinate lyase (ADSL) are dephosphorylated derivatives of SAICA ribotide (SAICAR) and adenylosuccinate (S-AMP), which catalyzes an important reaction in the de novo pathway of purine biosynthesis. ADSL catalyzes two distinct reactions in the synthesis of purine nucleotides, both of which involve the _-elimination of fumarate to produce either aminoimidazole carboxamide ribotide from SAICAR or AMP from S-AMP. The Adenylosuccinate lyase deficiency is a rare autosomal recessive metabolic disorder characterized by the present of SAICA riboside and succinyladenosine (S-Ado). ADSL defect in different patients is often caused by different mutations to the enzyme. |
偶联 | Unconjugated |
免疫原 | Recombinant Human Adenylosuccinate Lyase Protein (TMPY-02552) |
抗原种属 | Human |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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