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Anti-Adenylate cyclase 1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Adenylate cyclase 1。Anti-Adenylate cyclase 1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-Adenylate cyclase 1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Adenylate cyclase 1。Anti-Adenylate cyclase 1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,170 | 5日内发货 | |
100 μL | ¥ 1,975 | 5日内发货 | |
200 μL | ¥ 2,780 | 5日内发货 |
产品描述 | Anti-Adenylate cyclase 1 Polyclonal Antibody is a Rabbit antibody targeting Adenylate cyclase 1. Anti-Adenylate cyclase 1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
交叉反应 | Human,Rat (predicted:Mouse) |
验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Adenylate cyclase 1) Polyclonal Antibody, Unconjugated (TMAB-00060) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: MCF-7 Cell (Human) lysate at 30 μg Primary: Anti-ADCY1 (TMAB-00060) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 123 kDa Observed band size: 123 kDa 3. Sample: A549 Cell (Human) Lysate at 30 μg Primary: Anti-ADCY1 (TMAB-00060) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 123 kDa Observed band size: 123 kDa |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Membrane; Multi-pass membrane protein. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | The membrane bound adenylyl cyclases (ACs) represent one of the major families of effector enzymes for G protein coupled receptors. Eight human AC isoforms (AC-1 through AC-4) have been identified up to now and genes for at least nine adenylate cyclase (AC-1-AC-9) have been cloned characterized and sequenced. This is a calmodulin-sensitive adenylyl cyclase which may be involved in regulatory processes in the central nervous system. It may play a role in neuronal plasticity, memory acquisition and learning and is expressed predominantly in the brain, retina and adrenal medulla. |
免疫原 | KLH conjugated synthetic peptide: human adenylate cyclase type I |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Integration of energy metabolism,Integration of energy,Cancer,Regulation,Regulators |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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