Anti-ADAMTS5 Polyclonal Antibody is a Rabbit antibody targeting ADAMTS5. Anti-ADAMTS5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit)

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Aggrecanase-2/ADAMTS5 Polyclonal Antibody, Unconjugated (TMAB-00057) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Lane 1: Mouse Placenta tissue lysates
Lane 2: Mouse Spleen tissue lysates
Lane 3: Human U87MG cell lysates
Lane 4: Human U251 cell lysates
Lane 5: Human SH-SY5Y cell lysates
Lane 6: Human HeLa cell lysates
Primary: Anti-ADAMTS5 (TMAB-00057) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000

Polyclonal

KLH conjugated synthetic peptide: human ADAMTS5

Human

Aggrecan,MMP,ADAM Protein Family,ADAM TS