Anti-ACVR2B Polyclonal Antibody is a Rabbit antibody targeting ACVR2B. Anti-ACVR2B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Activin Receptor Type IIB) Polyclonal Antibody, Unconjugated (TMAB-00052) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
K562 (Human) Cell Lysate at 30 μg
293t (Human) Cell Lysate at 30 μg
Primary: Anti-Activin Receptor Type IIB (TMAB-00052) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
3. Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Mouse Muscle tissue lysates
Lane 3: Mouse Testis tissue lysates
Lane 4: Mouse Kidney tissue lysates
Lane 5: Human K562 cell lysates
Lane 6: Human SH-SY5Y cell lysates
Lane 7: Human Jurkat cell lysates
Lane 8: Human MOLT4 cell lysates
Primary: Anti-Activin Receptor Type IIB (TMAB-00052) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 60 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human ACVR2B/ACTR-IIB

Human

Actin Binding Proteins,Other Kinases,Surface Molecules