Anti-Actin, alpha skeletal muscle Antibody (8U812) is a Mouse antibody targeting Actin, alpha skeletal muscle. Anti-Actin, alpha skeletal muscle Antibody (8U812) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

8U812

Human,Rat (predicted:Mouse,Cow,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Actin, alpha skeletal muscle) Monoclonal Antibody, Unconjugated (TMAB-02258) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Actin, alpha skeletal muscle) Monoclonal Antibody, Unconjugated (TMAB-02258) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Actin, alpha skeletal muscle) Monoclonal Antibody, Unconjugated (TMAB-02258) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
4. 25 μg total protein per lane of various lysates (see on figure) probed with Actin, alpha skeletal muscle monoclonal antibody, unconjugated (TMAB-02258) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
5. Paraformaldehyde-fixed, paraffin embedded Rat Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Actin, alpha skeletal muscle Monoclonal Antibody, Unconjugated (TMAB-02258) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.
6. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Actin, alpha skeletal muscle Monoclonal Antibody, Unconjugated (TMAB-02258) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.

WB: 1:500-2000; IHC-P: 1:200-800; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

KLH conjugated synthetic peptide: human ACTA1

Human

ACTA1

Actin, alpha skeletal muscle

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.