Anti-ACSS2 Antibody (1N487) is a Rabbit antibody targeting ACSS2. Anti-ACSS2 Antibody (1N487) can be used in WB,ICC/IF,FCM.

IgG

1N487

Human,Rat (predicted:Mouse)

1. 25 μg total protein per lane of various lysates (see on figure) probed with ACSS2 monoclonal antibody, unconjugated (TMAB-02249) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The HepG2 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-ACSS2 antibody (TMAB-02249, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1:50-100

Monoclonal

A synthesized peptide: human ACSS2

Human

ACSS2

Acetyl-coenzyme A synthetase, cytoplasmic