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Anti-acnB Antibody (2I265)
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产品编号 TMAY-02485
别名 yacJ, yacI
Anti-acnB Antibody (2I265) 是一种 Mouse 抗体，靶向 acnB。Anti-acnB Antibody (2I265) 可用于 WB,ELISA。
Anti-acnB Antibody (2I265)
331 Anti-acnB Antibody (2I265)
还可以产品编号 TMAY-02485 别名 yacJ, yacI
Anti-acnB Antibody (2I265) 是一种 Mouse 抗体，靶向 acnB。Anti-acnB Antibody (2I265) 可用于 WB,ELISA。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 20 μL | ¥ 780 | 5日内发货 | |
| 100 μL | ¥ 2,289 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-acnB Antibody (2I265) is a Mouse antibody targeting acnB. Anti-acnB Antibody (2I265) can be used in WB,ELISA. |
| 别名 | yacJ, yacI |
| Ig Type | Mouse IgG1 |
| 克隆号 | 2I265 |
| 交叉反应 | E. coli |
| 应用 | WB,ELISA |
| 推荐剂量 | WB: his antibody can be used at 1:500-1:1000 with the appropriate secondary reagents to detect E.coli AcnB in WB. Using a DAB detection system, the detection limit for E.coli AcnB is approximately 4 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.; ELISA: 1:1000-1:2000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant E. coli acnB (TMPY-02375; P36683; Met 1-Val 865). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Escherichia coli contains two major aconitases (Acns), AcnA and AcnB. They are distantly related monomeric Fe-S proteins that contain different arrangements of four structural domains. acnA is specifically subject to SoxRS-mediated activation, whereas acnB encodes the major aconitase that is synthesized earlier in the growth cycle than AcnA. It is concluded that AcnB is the major citric acid cycle enzyme. Aconitate hydratase 2 (acnB) catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate, thus it functions as the major citric-acid-cycle enzyme during exponential growth. Escherichia coli acnB serves as either an enzymic catalyst or a mRNA-binding post-transcriptional regulator, depending on the status of its iron sulfur cluster. AcnB represents a large, distinct group of Gram-negative bacterial aconitases that have an altered domain organization relative to mitochondrial aconitase and other aconitases. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein: E. coli acnB protein (TMPY-02375) |
| 抗原种属 | E. coli |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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