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Anti-Acetylcholinesterase Polyclonal Antibody
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产品编号 TMAB-00034
Anti-Acetylcholinesterase Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Acetylcholinesterase。Anti-Acetylcholinesterase Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-Acetylcholinesterase Polyclonal Antibody
Anti-Acetylcholinesterase Polyclonal Antibody
还可以产品编号 TMAB-00034
Anti-Acetylcholinesterase Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Acetylcholinesterase。Anti-Acetylcholinesterase Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,975 | 5日内发货 | |
| 200 μL | ¥ 2,785 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-Acetylcholinesterase Polyclonal Antibody is a Rabbit antibody targeting Acetylcholinesterase. Anti-Acetylcholinesterase Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse (predicted:Rat,Dog,Cow,Horse) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with ACHE Polyclonal Antibody, Unconjugated (TMAB-00034) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. 2. Sample: Lane 1: Human K562 cell lysates Lane 2: Human U-2 OS cell lysates Lane 3: Human MCF-7 cell lysates Lane 4: Human SH-SY5Y cell lysates Primary: Anti-ACHE (TMAB-00034) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kDa Observed band size: 68 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cell junction, synapse. Secreted. Cell membrane; Peripheral membrane protein. Isoform T: Nucleus. Note=Only observed in apoptotic nuclei. Isoform H: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. |
| 免疫原 | KLH conjugated synthetic peptide: human AchE |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Cardiac arrhythmias,Acetylcholine |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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