Anti-ABI3BP Polyclonal Antibody is a Rabbit antibody targeting ABI3BP. Anti-ABI3BP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Rabbit)

1. Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-ABI3BP Polyclonal Antibody, Unconjugated (TMAB-00027) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Placenta (Mouse) Lysate at 40 μg
Hela (Human) Cell Lysate at 30 μg
Primary: Anti-ABI3BP (TMAB-00027) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 116 kDa
Observed band size: 116 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human ABI3BP

Human