Anti-ABI3 Polyclonal Antibody is a Rabbit antibody targeting ABI3. Anti-ABI3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Cow,Rabbit,Sheep)

1. 25 μg total protein per lane of various lysates (see on figure) probed with ABI3 polyclonal antibody, unconjugated (TMAB-02173) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. 25 μg total protein per lane of various lysates (see on figure) probed with ABI3 polyclonal antibody, unconjugated (TMAB-02173) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
3. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-ABI3 Polyclonal Antibody, Unconjugated (TMAB-02173) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human ABI3

Human

ABI3

ABI gene family member 3

May inhibit tumor metastasis. In vitro, reduces cell motility.