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顶部生物活性储存和溶解信息计算器

Anti-ABHD14B Antibody (9W382)

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Anti-ABHD14B Antibody (9W382)
产品编号 TMAY-02667
别名 HEL-S-299, CIB, abhydrolase domain containing 14B

Anti-ABHD14B Antibody (9W382) 是一种 Rabbit 抗体，靶向 ABHD14B。Anti-ABHD14B Antibody (9W382) 可用于 WB,IP。

Anti-ABHD14B Antibody (9W382)
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Anti-ABHD14B Antibody (9W382)

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Anti-ABHD14B Antibody (9W382)
产品编号 TMAY-02667 别名 HEL-S-299, CIB, abhydrolase domain containing 14B

Anti-ABHD14B Antibody (9W382) 是一种 Rabbit 抗体，靶向 ABHD14B。Anti-ABHD14B Antibody (9W382) 可用于 WB,IP。

规格价格库存数量
100 μL
¥ 2,294
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产品介绍

生物活性
产品描述
Anti-ABHD14B Antibody (9W382) is a Rabbit antibody targeting ABHD14B. Anti-ABHD14B Antibody (9W382) can be used in WB,IP.
别名HEL-S-299, CIB, abhydrolase domain containing 14B
Ig Type
Rabbit IgG
克隆号
9W382
交叉反应
Human
验证活性
1. Anti-ABHD14B rabbit monoclonal antibody at 1:500 dilution.
-Lane A: Jurkat Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:22 kDa.
-Observed band size:23 kDa.
2. ABHD14B was immunoprecipitated using:
-Lane A:0.5 mg Jurkat Whole Cell Lysate.
-Lane B:0.5 mg Hela Whole Cell Lysate
-0.5 µL anti-ABHD14B rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-ABHD14B rabbit monoclonal antibody, at 1:500 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 25 kDa.
-Observed band size: 25 kDa
应用
WB,IP
推荐剂量
WB: 1:500-1:1000; IP: 0.2-1 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human ABHD14B (rh ABHD14B; TMPY-02978; Q96IU4-1; Met1-Gln210).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景ABHD14B belongs to the AB hydrolase superfamily, ABHD14 family. It can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocyte, heart, placenta, lung, liver, skeletal muscle, pancreas and kidney. ABHD14B has hydrolase activity towards p-nitrophenyl butyrate (in vitro) and may interact with TAF1. It may activate transcription. Recombinant human ABHD14B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. ABHD14B contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. In molecular biology, the alpha/beta hydrolase fold is common to a number of hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The Ab hydrolase domain containing gene subfamily is comprised of 15 mostly uncharacterized members.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Human ABHD14B protein (TMPY-02978)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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关键词

HEL-S299HEL-S 299

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