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Anti-ABHD14B Antibody (9W382)
还可以产品编号 TMAY-02667
Anti-ABHD14B Antibody (9W382) 是一种 Rabbit 抗体，靶向 ABHD14B。Anti-ABHD14B Antibody (9W382) 可用于 WB,IP。
Anti-ABHD14B Antibody (9W382)
Anti-ABHD14B Antibody (9W382)
还可以产品编号 TMAY-02667
Anti-ABHD14B Antibody (9W382) 是一种 Rabbit 抗体，靶向 ABHD14B。Anti-ABHD14B Antibody (9W382) 可用于 WB,IP。
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抗原信息
| 产品描述 | Anti-ABHD14B Antibody (9W382) is a Rabbit antibody targeting ABHD14B. Anti-ABHD14B Antibody (9W382) can be used in WB,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 9W382 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-ABHD14B rabbit monoclonal antibody at 1:500 dilution. -Lane A: Jurkat Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:22 kDa. -Observed band size:23 kDa. 2. ABHD14B was immunoprecipitated using: -Lane A:0.5 mg Jurkat Whole Cell Lysate. -Lane B:0.5 mg Hela Whole Cell Lysate -0.5 µL anti-ABHD14B rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-ABHD14B rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 25 kDa. -Observed band size: 25 kDa |
| 应用 | WB,IP |
| 推荐剂量 | WB: 1:500-1:1000; IP: 0.2-1 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human ABHD14B (rh ABHD14B; TMPY-02978; Q96IU4-1; Met1-Gln210). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | ABHD14B belongs to the AB hydrolase superfamily, ABHD14 family. It can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocyte, heart, placenta, lung, liver, skeletal muscle, pancreas and kidney. ABHD14B has hydrolase activity towards p-nitrophenyl butyrate (in vitro) and may interact with TAF1. It may activate transcription. Recombinant human ABHD14B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. ABHD14B contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. In molecular biology, the alpha/beta hydrolase fold is common to a number of hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The Ab hydrolase domain containing gene subfamily is comprised of 15 mostly uncharacterized members. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human ABHD14B protein (TMPY-02978) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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