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Anti-ABHD14B Antibody (2N426) 是一种 Rabbit 抗体,靶向 ABHD14B。Anti-ABHD14B Antibody (2N426) 可用于 ELISA,ICC/IF。
Anti-ABHD14B Antibody (2N426) 是一种 Rabbit 抗体,靶向 ABHD14B。Anti-ABHD14B Antibody (2N426) 可用于 ELISA,ICC/IF。
产品描述 | Anti-ABHD14B Antibody (2N426) is a Rabbit antibody targeting ABHD14B. Anti-ABHD14B Antibody (2N426) can be used in ELISA,ICC/IF. |
Ig Type | Monoclonal Rabbit IgG |
克隆号 | 2N426 |
交叉反应 | Human |
验证活性 | Immunofluorescence staining of ABHD14B in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human ABHD14B monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to nucleus. |
应用 | ELISA,ICC/IF |
推荐剂量 | ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human ABHD14B (rh ABHD14B; TMPY-02978; Q96IU4-1; Met1-Gln210). |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
研究背景 | ABHD14B belongs to the AB hydrolase superfamily, ABHD14 family. It can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocyte, heart, placenta, lung, liver, skeletal muscle, pancreas and kidney. ABHD14B has hydrolase activity towards p-nitrophenyl butyrate (in vitro) and may interact with TAF1. It may activate transcription. Recombinant human ABHD14B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. ABHD14B contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. In molecular biology, the alpha/beta hydrolase fold is common to a number of hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The Ab hydrolase domain containing gene subfamily is comprised of 15 mostly uncharacterized members. |
偶联 | Unconjugated |
免疫原 | Recombinant Human ABHD14B Protein (TMPY-02978) |
抗原种属 | Human |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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