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Anti-ABCG2 Polyclonal Antibody

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Anti-ABCG2 Polyclonal Antibody
产品编号 TMAB-00024
别名 UAQTL1, Placenta specific MDR protein, Placenta specific ATP binding cassette transporter, MXR1, MXR, Multi drug resistance efflux transport ATP binding cassette sub family G(WHITE) member 2, MRX, Mitoxantrone resistance associated protein, MGC102821, GOUT1, EST157481, CDw338 antigen, CDw338, CD338, Breast cancer resistance protein, BMDP, BCRP1, ATP-binding cassette sub-family G member 2, ATP binding cassette transporter G2, ATP binding cassette sub family G(WHITE) member 2, ABCP, ABCG 2, ABC15

Anti-ABCG2 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 ABCG2。Anti-ABCG2 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,ELISA。

Anti-ABCG2 Polyclonal Antibody

Anti-ABCG2 Polyclonal Antibody

Rating icon 还可以
Anti-ABCG2 Polyclonal Antibody
产品编号 TMAB-00024 别名 UAQTL1, Placenta specific MDR protein, Placenta specific ATP binding cassette transporter, MXR1, MXR, Multi drug resistance efflux transport ATP binding cassette sub family G(WHITE) member 2, MRX, Mitoxantrone resistance associated protein, MGC102821, GOUT1, EST157481, CDw338 antigen, CDw338, CD338, Breast cancer resistance protein, BMDP, BCRP1, ATP-binding cassette sub-family G member 2, ATP binding cassette transporter G2, ATP binding cassette sub family G(WHITE) member 2, ABCP, ABCG 2, ABC15

Anti-ABCG2 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 ABCG2。Anti-ABCG2 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,ELISA。

规格价格库存数量
50 μL
¥ 1,170
5日内发货
100 μL
¥ 1,965
5日内发货
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产品描述
Anti-ABCG2 Polyclonal Antibody is a Rabbit antibody targeting ABCG2. Anti-ABCG2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
别名UAQTL1, Placenta specific MDR protein, Placenta specific ATP binding cassette transporter, MXR1, MXR, Multi drug resistance efflux transport ATP binding cassette sub family G(WHITE) member 2, MRX, Mitoxantrone resistance associated protein, MGC102821, GOUT1, EST157481, CDw338 antigen, CDw338, CD338, Breast cancer resistance protein, BMDP, BCRP1, ATP-binding cassette sub-family G member 2, ATP binding cassette transporter G2, ATP binding cassette sub family G(WHITE) member 2, ABCP, ABCG 2, ABC15
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-ABCG2/CD338 Polyclonal Antibody, Unconjugated (TMAB-00024) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-ABCG2/CD338 Polyclonal Antibody, Unconjugated (TMAB-00024) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
Lane 1: Mouse Placenta tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Mouse Lung tissue lysates
Lane 4: Rat Placenta tissue lysates
Lane 5: Rat Cerebrum tissue lysates
Lane 6: Rat Lung tissue lysates
Lane 7: Human MCF-7 cell lysates
Lane 8: Human HepG2 cell lysates
Lane 9: Human HUVEC cell lysates
Primary: Anti-ABCG2 (TMAB-00024) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 82 kDa
应用
WB
IHC-P
IHC-Fr
IF
ELISA
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cell membrane; Multi-pass membrane protein.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景An ABC transporter. Allows efflux of Hoechst dye, a property that has been used to separate bone marrow side population cells, which express BCRP/ABCG2. Appears to play a major role in the multidrug resistance phenotype of a specific MCF-7 breast cancer cell line. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123. Breast Cancer Resistance Protein (BCRP) is a 70 kDa ATP-Binding Cassette membrane transport protein involved in multidrug resistance. BCRP may be over-expressed in cancer cell lines selected with doxorubicin / verapamil, topotecan or mitoxantrone.
抗原信息
免疫原
KLH conjugated synthetic peptide: human ABCG2
抗原种属
Human
基因ID
9429
Uniprot ID
Q9UNQ0
研究领域
MRP-related proteins,Drug metabolism,Drug metabolism,ATPases,Surface Molecules,Surface Molecules,Tumor Associated
化学信息
分子量Theoretical: 72 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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