Anti-ABCG2 Polyclonal Antibody is a Rabbit antibody targeting ABCG2. Anti-ABCG2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.

IgG

Human,Mouse,Rat

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-ABCG2/CD338 Polyclonal Antibody, Unconjugated (TMAB-00024) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-ABCG2/CD338 Polyclonal Antibody, Unconjugated (TMAB-00024) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
Lane 1: Mouse Placenta tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Mouse Lung tissue lysates
Lane 4: Rat Placenta tissue lysates
Lane 5: Rat Cerebrum tissue lysates
Lane 6: Rat Lung tissue lysates
Lane 7: Human MCF-7 cell lysates
Lane 8: Human HepG2 cell lysates
Lane 9: Human HUVEC cell lysates
Primary: Anti-ABCG2 (TMAB-00024) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 82 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000

Polyclonal

KLH conjugated synthetic peptide: human ABCG2

Human

MRP-related proteins,Drug metabolism,Drug metabolism,ATPases,Surface Molecules,Surface Molecules,Tumor Associated