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顶部生物活性储存和溶解信息计算器

Anti-ABCG1 Polyclonal Antibody

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Anti-ABCG1 Polyclonal Antibody
产品编号 TMAB-00023

Anti-ABCG1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 ABCG1。Anti-ABCG1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-ABCG1 Polyclonal Antibody

Anti-ABCG1 Polyclonal Antibody

Rating icon 还可以
Anti-ABCG1 Polyclonal Antibody
产品编号 TMAB-00023

Anti-ABCG1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 ABCG1。Anti-ABCG1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,175
5日内发货
100 μL
¥ 1,960
5日内发货
200 μL
¥ 2,780
5日内发货
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产品介绍

生物活性
产品描述
Anti-ABCG1 Polyclonal Antibody is a Rabbit antibody targeting ABCG1. Anti-ABCG1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
交叉反应
Human,Mouse,Rat (predicted:Dog)
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with ABCG1 Polyclonal Antibody, Unconjugated (TMAB-00023) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-ABCG1 (TMAB-00023) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 76 kDa
Observed band size: 76 kDa
3. Paraformaldehyde-fixed, paraffin embedded (Human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ABCG1) Polyclonal Antibody, Unconjugated (TMAB-00023) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ABCG1) Polyclonal Antibody, Unconjugated (TMAB-00023) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
应用
WB
IHC-P
IHC-Fr
IF
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Endoplasmic reticulum membrane. Golgi apparatus membrane. Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types. Six alternative splice variants have been identified.
抗原信息
免疫原
KLH conjugated synthetic peptide: human ABCG1
抗原种属
Human
基因ID
9619
Uniprot ID
P45844
研究领域
Metabolism of lipids and lipoproteins,Lipid transport,Lipoprotein metabolism,Cholesterol Metabolism,Lipoproteins/Apolipoproteins,Cholesterol Metabolism,Lipid metabolism,Lipoprotein metabolism,Cancer,Heart disease,Astrocyte marker,Lipid metabolism
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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