Anti-ABCD3 Antibody (1U962) is a Rabbit antibody targeting ABCD3. Anti-ABCD3 Antibody (1U962) can be used in FCM, ICC/IF.

IgG

1U962

Human (predicted:Mouse,Rat)

1. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (ABCD3) monoclonal Antibody, unconjugated (TMAB-02154) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-ABCD3 antibody (TMAB-02154, 1: 100）; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (BF488): 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; ICC/IF=1:50-200

Monoclonal

A synthesized peptide: human ABCD3

Human

ABCD3

ATP-binding cassette sub-family D member 3

Probable transporter. The nucleotide-binding fold acts as an ATP-binding subunit with ATPase activity.