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Anti-AACT Antibody (1F833)

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货号 TMAB-02123

别名 SERPINA3, serpin family A member 3, Serpin A3, GIG25, GIG24, Cell growth-inhibiting gene 24/25 protein, alpha-1-antichymotrypsin, ACT, AACT_HUMAN

Anti-AACT Antibody (1F833) 是一种 Rabbit 抗体，靶向 AACT。Anti-AACT Antibody (1F833) 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-AACT Antibody (1F833)

Anti-AACT Antibody (1F833)

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Rating icon 还可以
货号 TMAB-02123 别名 SERPINA3, serpin family A member 3, Serpin A3, GIG25, GIG24, Cell growth-inhibiting gene 24/25 protein, alpha-1-antichymotrypsin, ACT, AACT_HUMAN

Anti-AACT Antibody (1F833) 是一种 Rabbit 抗体，靶向 AACT。Anti-AACT Antibody (1F833) 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
25 μL
¥ 785
5日内发货
50 μL
¥ 1,380
5日内发货
100 μL
¥ 2,490
5日内发货
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产品介绍

生物活性
产品描述
Anti-AACT Antibody (1F833) is a Rabbit antibody targeting AACT. Anti-AACT Antibody (1F833) can be used in WB,IHC-P,IHC-Fr,IF.
别名SERPINA3, serpin family A member 3, Serpin A3, GIG25, GIG24, Cell growth-inhibiting gene 24/25 protein, alpha-1-antichymotrypsin, ACT, AACT_HUMAN
Ig Type
IgG
克隆号
1F833
反应种属
Human
验证活性
1. 25 μg total protein per lane of various lysates (see on figure) probed with AACT monoclonal antibody, unconjugated (TMAB-02123) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AACT Monoclonal Antibody, Unconjugated (TMAB-02123) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AACT Monoclonal Antibody, Unconjugated (TMAB-02123) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AACT Monoclonal Antibody, Unconjugated (TMAB-02123) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AACT Monoclonal Antibody, Unconjugated (TMAB-02123) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AACT Monoclonal Antibody, Unconjugated (TMAB-02123) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human tonsils; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AACT Monoclonal Antibody, Unconjugated (TMAB-02123) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.
应用WBIHC-PIHC-FrIF
推荐剂量
WB: 1:2000-10000; IHC-P: 1:200-800; IHC-Fr: 1:200-800; IF: 1:200-800
抗体种类
Monoclonal
宿主来源Rabbit
亚细胞定位Secreted.
组织特异性Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.
构建方式Recombinant Antibody
纯化方式Protein A purified
性状Lyophilized or Liquid
缓冲液PBS, Glycerol, BSA.
浓度1mg/ml
研究背景Alpha 1-Antichymotrypsin, a member of the serine proteinase inhibitor (serpin) family, inhibits neutrophil proteinase cathepsin G and mast cell chymases, and protects the lower respiratory tract from damage by proteolytic enzymes. It contains a reactive centre loop, which interacts with cognate proteinases, resulting in loop cleavage and a major conformational change. Recently, alpha 1-antichymotrypsin has been identified as a major constituent of the neurofibrillary plaques associated with Alzheimers disease, and in vitro studies have shown that it enhances the rate of amyloid-fibril formation. These observations and recent genetic evidence suggest that alpha 1-antichymotrypsin is important in the pathogenesis of Alzheimers disease.
抗原信息
免疫原
Full length native Protein
抗原种属
Human
基因名称
SERPINA3
基因ID
12
蛋白名称
alpha-1-antichymotrypsin
Uniprot ID
P01011
功能
Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
化学信息
分子量Theoretical: 45 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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