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Anti-A Raf Polyclonal Antibody

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货号 TMAB-02116

别名 v raf oncogene homolog 1(murine sarcoma 3611 virus), v raf murine sarcoma 3611 viral oncogene homolog 1, v raf murine sarcoma 3611 viral oncogene homolog, Ras binding protein DA Raf, RAFA1, RAFA 1, Raf1, Proto oncogene Pks, PKS2, PKS 2, PKS, Oncogene PKS2, Oncogene Araf1, ARAF1, ARaf proto oncogene serine/threonine protein kinase, ARAF 1, A-Raf, ARAF, A Raf proto oncogene serine/threonine protein kinase, A raf 1

Anti-A Raf Polyclonal Antibody 是一种 Rabbit 抗体，靶向 A Raf。Anti-A Raf Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-A Raf Polyclonal Antibody

Anti-A Raf Polyclonal Antibody

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货号 TMAB-02116 别名 v raf oncogene homolog 1(murine sarcoma 3611 virus), v raf murine sarcoma 3611 viral oncogene homolog 1, v raf murine sarcoma 3611 viral oncogene homolog, Ras binding protein DA Raf, RAFA1, RAFA 1, Raf1, Proto oncogene Pks, PKS2, PKS 2, PKS, Oncogene PKS2, Oncogene Araf1, ARAF1, ARaf proto oncogene serine/threonine protein kinase, ARAF 1, A-Raf, ARAF, A Raf proto oncogene serine/threonine protein kinase, A raf 1

Anti-A Raf Polyclonal Antibody 是一种 Rabbit 抗体，靶向 A Raf。Anti-A Raf Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,165
5日内发货
100 μL
¥ 1,960
5日内发货
200 μL
¥ 2,795
5日内发货
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产品介绍

生物活性
产品描述
Anti-A Raf Polyclonal Antibody is a Rabbit antibody targeting A Raf. Anti-A Raf Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
别名v raf oncogene homolog 1(murine sarcoma 3611 virus), v raf murine sarcoma 3611 viral oncogene homolog 1, v raf murine sarcoma 3611 viral oncogene homolog, Ras binding protein DA Raf, RAFA1, RAFA 1, Raf1, Proto oncogene Pks, PKS2, PKS 2, PKS, Oncogene PKS2, Oncogene Araf1, ARAF1, ARaf proto oncogene serine/threonine protein kinase, ARAF 1, A-Raf, ARAF, A Raf proto oncogene serine/threonine protein kinase, A raf 1
Ig Type
IgG
反应种属
Human,Rat (predicted:Mouse)
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (A Raf) Polyclonal Antibody, Unconjugated (TMAB-02116) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample:
293T (Human) Cell Lysate at 30 μg
Raji (Human) Cell Lysate at 30 μg
Primary: Anti-A Raf (TMAB-02116) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 65 kD
3. Sample:
Hela (Human) Cell Lysate at 30 μg
NIH/3T3 (Mouse) Cell Lysate at 30 μg
Primary: Anti-A Raf (TMAB-02116) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 65 kD
4. Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (A Raf) Polyclonal Antibody, Unconjugated (TMAB-02116) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
应用WBIHC-PIHC-FrIF
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
组织特异性Predominantly in urogenital tissues.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景The RAF family of signaling proteins has three isoforms: A RAF, RAF 1 and B RAF. These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway. RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain. This domain is conserved in A RAF and B RAF. 14-3-3 is also known to be an activator of RAFs. Activated RAF directly phosphorylates and activates MEK. A RAF is expressed at a very high concentration in urogenital and kidney tissues.
抗原信息
免疫原
KLH conjugated synthetic peptide: human A Raf/ARAF
抗原种属
Human
基因名称
ARAF
基因ID
369
蛋白名称
Serine/threonine-protein kinase A-Raf
Uniprot ID
P10398
功能
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus.
化学信息
分子量Theoretical: 68 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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