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别名 Trophoblast glycoprotein, TPBG_HUMAN, TPBG, M6P1, 5T4AG, 5T4 oncotrophoblast glycoprotein, 5T4 oncofetal trophoblast glycoprotein, 5T4 oncofetal antigen, 5T4
Anti-5T4 Antibody (6K317) 是一种 Rabbit 抗体,靶向 5T4。Anti-5T4 Antibody (6K317) 可用于 WB,IHC-P,IHC-Fr。
Anti-5T4 Antibody (6K317) 是一种 Rabbit 抗体,靶向 5T4。Anti-5T4 Antibody (6K317) 可用于 WB,IHC-P,IHC-Fr。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 25 μL | ¥ 790 | 5日内发货 | |
| 50 μL | ¥ 1,380 | 5日内发货 | |
| 100 μL | ¥ 2,485 | 5日内发货 |
| 产品描述 | Anti-5T4 Antibody (6K317) is a Rabbit antibody targeting 5T4. Anti-5T4 Antibody (6K317) can be used in WB,IHC-P,IHC-Fr. |
| 别名 | Trophoblast glycoprotein, TPBG_HUMAN, TPBG, M6P1, 5T4AG, 5T4 oncotrophoblast glycoprotein, 5T4 oncofetal trophoblast glycoprotein, 5T4 oncofetal antigen, 5T4 |
| Ig Type | IgG |
| 克隆号 | 6K317 |
| 反应种属 | Human |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 5T4 Monoclonal Antibody, Unconjugated (TMAB-02115) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 5T4 Monoclonal Antibody, Unconjugated (TMAB-02115) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 3. 25 μg total protein per lane of various lysates (see on figure) probed with 5T4 monoclonal antibody, unconjugated (TMAB-02115) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| 应用 | WBIHC-PIHC-Fr |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Membrane. |
| 组织特异性 | Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 浓度 | 1mg/ml |
| 研究背景 | TPBG is a 420 amino acid single-pass type I membrane protein expressed by all types of trophoblasts as early as 9 weeks of development. TPBG contains an N-terminal putative signal sequence, a 310-residue extracellular region, a membrane anchorage domain and a 44-amino acid cytoplasmic tail with a potential phosphorylation site. The extracellular region has seven potential N-glycosylation sites and seven leucine-rich repeats, which are located in two regions separated by a hydrophilic stretch. Suggested to be involved in cell adhesion, TPBG may also be associated with tumor growth and progression. |
| 免疫原 | KLH conjugated synthetic peptide: human TPBG/5T4 |
| 抗原种属 | Human |
| 基因名称 | Tpbg |
| 基因ID | |
| 蛋白名称 | Trophoblast glycoprotein |
| Uniprot ID |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |