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Anti-5HT7 Receptor Polyclonal Antibody 是一种 Rabbit 抗体,靶向 5HT7 Receptor。Anti-5HT7 Receptor Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-5HT7 Receptor Polyclonal Antibody 是一种 Rabbit 抗体,靶向 5HT7 Receptor。Anti-5HT7 Receptor Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,165 | 5日内发货 | |
100 μL | ¥ 1,975 | 5日内发货 | |
200 μL | ¥ 2,785 | 5日内发货 |
产品描述 | Anti-5HT7 Receptor Polyclonal Antibody is a Rabbit antibody targeting 5HT7 Receptor. Anti-5HT7 Receptor Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Sample: Hippocampus (Mouse) Lysate at 40 μg Hippocampus (Rat) Lysate at 40 μg Primary: Anti-5HT7 Receptor (TMAB-00013) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa 2. Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Cerebellum tissue lysates Lane 3: Rat Cerebrum tissue lysates Lane 4: Rat Cerebellum tissue lysates Primary: Anti-5HT7 Receptor (TMAB-00013) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 50 kDa 3. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (5HT7 Receptor) Polyclonal Antibody, Unconjugated (TMAB-00013) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (5HT7 Receptor) Polyclonal Antibody, Unconjugated (TMAB-00013) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (5HT7 Receptor) Polyclonal Antibody, Unconjugated (TMAB-00013) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Cell membrane; Multi-pass membrane protein. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | The neurotransmitter, serotonin, is thought to play a role in various cognitive and behavioral functions. The serotonin receptor encoded by this gene belongs to the superfamily of G protein-coupled receptors and the gene is a candidate locus for involvement in autistic disorder and other neuropsychiatric disorders. Three splice variants have been identified which encode proteins that differ in the length of their carboxy terminal ends. [provided by RefSeq, Jul 2008] |
免疫原 | KLH conjugated synthetic peptide: human 5HT7 Receptor/SR-7 |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Obesity,Serotonin Receptors |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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