Anti-5HT2C Receptor Polyclonal Antibody is a Rabbit antibody targeting 5HT2C Receptor. Anti-5HT2C Receptor Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse)

1. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with 5HTR2C Polyclonal Antibody, Unconjugated (TMAB-00010) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Lane 1: Human SH-SY5Y cell lysates
Lane 2: Human A431 cell lysates
Primary: Anti-5HT2C Receptor (TMAB-00010) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 59 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human 5HTR2C

Human

HTR2C

5-hydroxytryptamine receptor 2C

Obesity,Metabolism,Serotonin Receptors,GPCR

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.