Anti-5HT2B Receptor Polyclonal Antibody 2 is a Rabbit antibody targeting 5HT2B Receptor. Anti-5HT2B Receptor Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human

1. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (5HT2B Receptor) Polyclonal Antibody, Unconjugated (TMAB-02104) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human SH-SY5Y cell lysates
Lane 3: Human MCF-7 cell lysates
Lane 4: Human HeLa cell lysates
Primary: Anti-5HT2B Receptor (TMAB-02104) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 60 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human 5HT2B Receptor/SR-2B

Human

HTR2B

5-hydroxytryptamine receptor 2B

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Plays a role in the regulation of impulsive behavior.