Anti-5 MethylCytosine Polyclonal Antibody 2 is a Rabbit antibody targeting 5 MethylCytosine. Anti-5 MethylCytosine Polyclonal Antibody 2 can be used in FCM.

IgG

Species independent

1. Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-5 MethylCytosine antibody (TMAB-02094)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE Dilution: 1 μg /test.
Protocol: The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 1μg/Test

Polyclonal

KLH conjugated 5 MethylCytosine