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Anti-5-BrdU Antibody (4C731)
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产品编号 TMAB-00006
Anti-5-BrdU Antibody (4C731) 是一种 Mouse 抗体，靶向 5-BrdU。Anti-5-BrdU Antibody (4C731) 可用于 ICC/IF。
Anti-5-BrdU Antibody (4C731)
Anti-5-BrdU Antibody (4C731)
还可以产品编号 TMAB-00006
Anti-5-BrdU Antibody (4C731) 是一种 Mouse 抗体，靶向 5-BrdU。Anti-5-BrdU Antibody (4C731) 可用于 ICC/IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,160 | 5日内发货 | |
| 100 μL | ¥ 1,965 | 5日内发货 | |
| 200 μL | ¥ 2,785 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-5-BrdU Antibody (4C731) is a Mouse antibody targeting 5-BrdU. Anti-5-BrdU Antibody (4C731) can be used in ICC/IF. |
| Ig Type | IgG |
| 克隆号 | 4C731 |
| 交叉反应 | Species independent |
| 验证活性 | 4% Paraformaldehyde-fixed Hela (treated with 10uM BrdU for 24 hours) (H) cells; 2M HCL at RT for 20 min; Antibody incubation with (BrdU) monoclonal Antibody, unconjugated (TMAB-00006) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. |
| 应用 | |
| 推荐剂量 | ICC/IF: 1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Nuclear. |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein G purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis |
| 免疫原 | KLH conjugated Brdu |
| 研究领域 | Markers,DNA / Nucleotides,Soma marker,Replication |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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