keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
BCECF is a fluorescent probe commonly used to measure pH. It is a dual-excitation ratiometric pH indicator with a pKa of ~6.98. Measurements of pH are made by determining the ratio of emission intensity, detected at 535 nm when excited at 490 nm versus the emission intensity when excited at 440 nm.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
500 μg | ¥ 647 | 35日内发货 | ||
1 mg | ¥ 1,130 | 35日内发货 | ||
5 mg | ¥ 3,960 | 35日内发货 |
产品描述 | BCECF is a fluorescent probe commonly used to measure pH. It is a dual-excitation ratiometric pH indicator with a pKa of ~6.98. Measurements of pH are made by determining the ratio of emission intensity, detected at 535 nm when excited at 490 nm versus the emission intensity when excited at 440 nm. |
体外活性 | The staining is seen in the interior of hydrogenosomes in some instances. It is also observed by microscopy that the K+/H+ ionophore nigericin does not inhibit hydrogenosomes loading with BCECF. |
别名 | 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein |
分子量 | 1040.892 |
分子式 | C54H40O22 |
CAS No. | 85138-49-4 |
keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 62.5 mg/mL (120.09 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 0.9607 mL | 4.8036 mL | 9.6071 mL | 24.0179 mL |
5 mM | 0.1921 mL | 0.9607 mL | 1.9214 mL | 4.8036 mL | |
10 mM | 0.0961 mL | 0.4804 mL | 0.9607 mL | 2.4018 mL | |
20 mM | 0.048 mL | 0.2402 mL | 0.4804 mL | 1.2009 mL | |
50 mM | 0.0192 mL | 0.0961 mL | 0.1921 mL | 0.4804 mL | |
100 mM | 0.0096 mL | 0.048 mL | 0.0961 mL | 0.2402 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
BCECF 85138-49-4 Others 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein Inhibitor inhibitor inhibit