8-Azido-ATP is a nucleotide analog that demonstrates photo-reactivity properties. It offers utility in the precise labeling and identification of proteins, particularly those involved in DNA-dependent RNA polymerase activity.

8-Azido-ATP can be used to identify the viral RNA polymerase on the basis of the ability of the analog to inhibit transcription activity associated with rotavirus particles on exposure to UV light[1]. 8-Azido-ATP (azido-ATP) decreases viral transcription in a dose-dependent manner[1]. 8-Azido-ATP blocks Kir6.2ΔC26 currents rather less potently than ATP, half-maximal inhibition (Ki) occurring at 2.8 ± 0.4 mM (n = 6) compared with 172 ± 7 mM for ATP (n = 6). The Hill coefficients were 0.9 ± 0.2 for 8-azido-ATP and 1.3 ± 0.1 for ATP[2].