2,5-Dimethylchromone-7-O-β-D-glucopyranoside is an orally active chromone glycoside found in the underground parts of Rheum australe. It exhibits the ability to scavenge DPPH free radicals with an IC50 value of 66.9 μM. The compound inhibits lipid peroxidation by donating hydrogen atoms through its phenolic hydroxyl groups, making it a promising candidate for research related to oxidative stress-associated diseases, such as inflammation and dermatological conditions.