18:0,18:1 PS is a predominant phosphatidylserine subtype found in cells, exosomes, and HIV particles, as well as being abundant in the brain. It plays a crucial role in maintaining membrane structure, lipid raft organization, and intracellular transport. This compound interacts with very long-chain sphingolipids in a cholesterol-dependent manner to mediate coupling between membrane leaflets and can induce the aggregation of glycosylphosphatidylinositol-anchored proteins. Furthermore, clusters formed by 18:0,18:1 PS and cholesterol not only support the proper distribution of cholesterol in the bilayer membrane but also protect it from oxidative damage effectively.